Potulutsa matani opitilira 700,000 pachaka, glyphosate ndiye mankhwala opha udzu omwe amagwiritsidwa ntchito kwambiri padziko lonse lapansi.Kukana udzu komanso ziwopsezo zomwe zingawononge chilengedwe komanso thanzi la anthu chifukwa cha nkhanza za glyphosate zakopa chidwi chachikulu.
Pa Meyi 29th, gulu la Pulofesa Guo Ruiting lochokera ku State Key Laboratory of Biocatalysis and Enzyme Engineering, lomwe linakhazikitsidwa pamodzi ndi School of Life Sciences of Hubei University ndi dipatimenti ya zigawo ndi unduna, idasindikiza kafukufuku waposachedwa mu Journal of Hazardous Materials, kusanthula. kusanthula koyamba kwa udzu wa barnyard.(Udzu woyipa wa paddy) -wopangidwa ndi aldo-keto reductase AKR4C16 ndi AKR4C17 imathandizira momwe glyphosate imawonongeka, ndikuwongolera kwambiri kuwonongeka kwa glyphosate ndi AKR4C17 kudzera mukusintha kwa maselo.
Kukana kwa glyphosate.
Kuyambira pomwe idakhazikitsidwa m'ma 1970, glyphosate yakhala yotchuka padziko lonse lapansi, ndipo pang'onopang'ono yakhala yotsika mtengo kwambiri, yogwiritsidwa ntchito kwambiri komanso yotulutsa udzu wambiri.Zimayambitsa kusokonezeka kwa kagayidwe kachakudya m'zomera, kuphatikizapo namsongole, poletsa makamaka 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), puloteni yofunika kwambiri yomwe imakhudzidwa ndi kukula kwa zomera ndi kagayidwe kake.ndi imfa.
Choncho, kuswana glyphosate-resistant mbewu za transgenic ndi kugwiritsa ntchito glyphosate m'munda ndi njira yofunikira yothetsera udzu paulimi wamakono.
Komabe, ndikugwiritsa ntchito molakwika glyphosate ndikugwiritsa ntchito molakwika, udzu wambiri wasintha pang'onopang'ono ndikukulitsa kulolerana kwa glyphosate.
Kuphatikiza apo, mbewu zosinthidwa ndi glyphosate zosagwirizana ndi glyphosate sizingawononge glyphosate, zomwe zimapangitsa kuti glyphosate ikhale ndi mbewu, zomwe zimatha kufalikira mosavuta kudzera munjira yazakudya ndikuyika thanzi la munthu pachiwopsezo.
Chifukwa chake, ndikofunikira kuti mupeze majini omwe amatha kuwononga glyphosate, kuti muthe kulima mbewu za transgenic zolimbana ndi glyphosate ndi zotsalira zochepa za glyphosate.
Kuthetsa kapangidwe ka kristalo ndi njira yothandizira ma enzymes omwe amachokera ku glyphosate
Mu 2019, magulu ofufuza aku China ndi ku Australia adapeza ma aldo-keto reductase omwe amawononga glyphosate, AKR4C16 ndi AKR4C17, kwa nthawi yoyamba kuchokera ku udzu wosamva glyphosate.Atha kugwiritsa ntchito NADP+ ngati cofactor kuti awononge glyphosate kukhala nontoxic aminomethylphosphonic acid ndi glyoxylic acid.
AKR4C16 ndi AKR4C17 ndi ma enzymes oyamba owononga a glyphosate opangidwa ndi kusintha kwachilengedwe kwa zomera.Pofuna kuwunikanso momwe ma cell amawonongera glyphosate, gulu la Guo Ruiting linagwiritsa ntchito X-ray crystallography kusanthula ubale wapakati pa ma enzyme awiriwa ndi cofactor high.Mapangidwe ovuta a chigamulochi adawulula momwe amamangirira ma ternary complex a glyphosate, NADP+ ndi AKR4C17, ndipo adapereka malingaliro othandizira machitidwe a AKR4C16 ndi AKR4C17-mediated glyphosate degradation.
Kapangidwe ka AKR4C17/NADP+/glyphosate complex ndi reaction mechanism of glyphosate degradation.
Kusintha kwa mamolekyulu kumathandizira kuwonongeka kwa glyphosate.
Atapeza mawonekedwe abwino amitundu itatu a AKR4C17/NADP+/glyphosate, gulu la Pulofesa Guo Ruiting linapezanso mapuloteni osinthika AKR4C17F291D ndi chiwonjezeko cha 70% pakuwonongeka kwa glyphosate kudzera kusanthula kapangidwe ka enzyme ndi kapangidwe koyenera.
Kuwunika kwa glyphosate-degrading zochita za AKR4C17 mutants.
"Ntchito yathu ikuwonetsa momwe ma cell a AKR4C16 ndi AKR4C17 amathandizira kuwonongeka kwa glyphosate, zomwe zimayala maziko ofunikira pakukonzanso kwa AKR4C16 ndi AKR4C17 kuti apititse patsogolo kuwonongeka kwawo kwa glyphosate."Wolemba wina wa pepalali, Pulofesa wina wa ku Hubei University, Dai Longhai, adati adapanga mapuloteni osinthika AKR4C17F291D omwe amatha kuwononga glyphosate, omwe amapereka chida chofunikira polima mbewu zamtundu wosagwirizana ndi glyphosate zotsalira zochepa za glyphosate komanso kugwiritsa ntchito mabakiteriya opangidwa ndi ma microbial engineering. kuwononga glyphosate m'chilengedwe.
Akuti gulu la Guo Ruiting lakhala likuchita nawo kafukufuku wokhudza kusanthula kapangidwe kake ndi kukambirana kwamakina a michere ya biodegradation, ma synthase a terpenoid, ndi mapuloteni opangira mankhwala azinthu zapoizoni komanso zovulaza chilengedwe.Li Hao, wofufuza wothandizana nawo Yang Yu ndi mphunzitsi Hu Yumei mgululi ndi omwe adalemba nawo pepalali, ndipo Guo Ruiting ndi Dai Longhai ndi olemba anzawo.
Nthawi yotumiza: Jun-02-2022