γ-Valactone imalimbikitsa kukula kwa mbande za phwetekere kudzera mu kusintha kwa transcriptome ndi mahomoni a zomera | Plant Molecular Biology

Pofuna kuchepetsa kudalira kwa ulimi pa feteleza wa mankhwala, chidwi cha mitundu yatsopano ya feteleza chikukulirakulira.olimbikitsa kukula kwa zomera omwe ali ndi chiyembekezo.Gamma-valactone, mankhwala osungunuka omwe amapangidwa ndi *S. stearothermiae* NRCB010, awonetsedwa kuti amalimbikitsa kukula kwa mbande za phwetekere pansi pa mikhalidwe yosasamalidwa. Chifukwa chake, tidafufuza zotsatira za kuchuluka kosiyanasiyana kwa gamma-valactone pakukula kwa mbande za phwetekere pansi pa mikhalidwe ya hydroponic ndi greenhouse, komanso tidayang'ana kusintha kofanana mu kuchuluka kwa ma transcription ndi mahomoni. Zotsatira zake zidawonetsa kuti chithandizo cha gamma-valactone chidathandizira kwambiri kukula kwa mbande za phwetekere, ndi kuchuluka kwabwino kwa 100 mg/L ndi 250 mg/L pansi pa mikhalidwe ya hydroponic ndi greenhouse, motsatana. Zomwe zili mu IAA-aspartic acid, IAA-glutamic acid,indole-3-acetamide, indole-3-acetonitrile, indole-3-butyric acid, ndi gibberellin m'masamba, tsinde, ndi mizu zinawonjezeka kwambiri. Kusanthula kwa Transcriptome kunawonetsa kuti pambuyo pa maola 6 a chithandizo cha γ-valerolactone, chiwerengero cha majini owonetsedwa mosiyana (DEGs) okhala ndi mawonekedwe owonjezereka chinaposa chiwerengero cha majini okhala ndi mawonekedwe ocheperako; komabe, mkhalidwe wosiyana unawonedwa pambuyo pa maola 24 a chithandizo. γ-valerolactone ikhoza kulimbikitsa kukula kwa mbande za phwetekere kudzera mu njira monga photosynthesis, plant hormone signaling, ndi secondary metabolite biosynthesis. Kafukufukuyu amapereka maziko a chiphunzitso cha kugwiritsa ntchito γ-valerolactone ngati chowongolera chatsopano cha kukula kwa zomera ndipo akuyika maziko opangira biostimulants zaulimi zothandiza kwambiri komanso zosawononga chilengedwe.

Kusanthula kwa morphological, transcriptomic ndi mahomoni kunawonetsa kuti γ-valerolactone imathandizira kukula kwa mbande za phwetekere ndikuwonjezera kuchuluka kwa mahomoni.
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Tikuthokoza kwambiri bungwe la High Performance Computing Center la Nanjing University of Technology chifukwa chotipatsa zida zogwiritsira ntchito makompyuta.
Kafukufukuyu adathandizidwa ndi National Natural Science Foundation of China (32172673 ndi 31972503) ndi Project of the Student Innovation and Entrepreneurship Training Platform of Nanjing University of Technology (2025DC0047, 2025DC1680, 2025DC1682).
Dipatimenti ya Biotechnology ndi Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, China
Jiangsu Provincial Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Collaborative Innovation Center for Atmospheric Environment and Equipment Technology, Sukulu ya Sayansi ndi Uinjiniya wa Zachilengedwe, Nanjing University of Information Science and Technology, Nanjing 210044, China
Conceptualization, Shanshan Li; Kusunga deta: Xiyue Sha, Shanshan Li, Hui Shen; Kusanthula kovomerezeka, Fangfang Ren ndi Xin Tao; Kupeza ndalama: Nan Gao, Weiye Chen, Xiyue Sha, ndi Zhuorui Li; Njira, Fangfang Ren ndi Weiye Chen; Utsogoleri wa polojekiti, Nan Gao; Kulemba - zolemba zoyambirira, Fangfang Ren ndi Hui Shen; Kulemba, kubwereza, ndi kukonza: Weishou Shen, Hui Shen, ndi Nan Gao.
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