Kodi pali kusiyana kotani pakati pa zeatin, Trans-zeatin ndi zeatin riboside? Kodi ntchito zawo ndi zotani?

Ntchito zazikulu

1. Limbikitsani kugawanika kwa maselo, makamaka kugawanika kwa cytoplasm;

2. Limbikitsani kusiyana kwa masamba. Mu chikhalidwe cha minofu, imagwirizana ndi auxin kuti iwononge kusiyanitsa ndi mapangidwe a mizu ndi masamba;

3. Limbikitsani chitukuko cha masamba ofananira nawo, kuchotsani kulamulira kwa apical, ndipo motero kumapangitsa kuti pakhale masamba ambiri obwera mu chikhalidwe cha minofu;

4. Kuchedwetsa kumera kwa masamba, kuchepetsa kuwonongeka kwa chlorophyll ndi mapuloteni;

5. Dulani kusowa kwa mbeu, sinthani kuwala kuti mukwaniritse kuwala kwa mbewu monga fodya;

6. Limbikitsani parthenocarpy mu zipatso zina;

7. Limbikitsani kupangika kwa masamba oyambira: kumapeto kwa masamba komanso mu mosses zina, zitha kulimbikitsa mapangidwe a masamba oyambira;

8. Kulimbikitsa mapangidwe mbatata tubers.

Zimangokhala ndi mawonekedwe a trans ndipo zimakhala ndi zotsatira zofanana ndizeatin, koma ndi ntchito yamphamvu.

Zotsatira zake ndizofanana kwambiri ndi anti-zeatin. Sikuti ali ndi ntchito zomwe tatchulazi za zeatin, komanso zimakhala ndi zotsatira zoyambitsa ma jini komanso machitidwe a metabolic.

Kugwiritsa Ntchito Njira

1. Limbikitsani kumera kwa callus (kuyenera kugwiritsidwa ntchito limodzi ndi auxin), ndende ya 1mg/L.

2. Limbikitsani kuika zipatso, 1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, utsi pa zipatso 10, 25, ndi masiku 40 mutatha maluwa.

3. Pamasamba a masamba, tsitsani pa 201 mg/L kuti masamba achedwe chikasu.

Kuphatikiza apo, kuchiza mbewu zina kumathandizira kumera; kuchiza pa siteji ya mmera kumalimbikitsa kukula.

1. Limbikitsani kumera kwa minofu ya callus (iyenera kugwiritsidwa ntchito limodzi ndi auxin), pamagulu a 1 ppm;

2. Limbikitsani kuyika kwa zipatso, 100 ppm ya cytokinin + 500 ppm ya GA3 + 20 ppm ya NAA, tsitsani zipatso 10, 25, ndi masiku 40 mutatha maluwa;

3. Kuchedwetsa chikasu masamba masamba, utsi 20 ppm;

1. Mu chikhalidwe cha minofu ya zomera, chiwerengero chofala cha anti-cytokinin nucleoside ndi 1 mg / mL kapena apamwamba.

2. Mu malamulo a kukula kwa zomera, ndende ya anti-cytokinin nucleoside nthawi zambiri imakhala 1 ppm mpaka 100 ppm, ndipo chigawo chenichenicho chimadalira pa ntchito yeniyeni ndi mitundu ya zomera. Mwachitsanzo, polimbikitsa kumera kwa minofu ya callus, anti-cytokinin nucleoside ndi 1 ppm, ndipo iyenera kugwiritsidwa ntchito limodzi ndi auxin.

3. Sungunulani anti-cytokinin nucleoside powder bwinobwino ndi 2-5 mL ya 1 M NaOH (kapena 1 M acetic acid kapena 1 M KOH), kenaka yikani madzi osungunuka kawiri kapena madzi a ultrapure kuti mukonzekere njira yosungirako 1 mg / mL kapena yowonjezereka. Sakanizani pamene mukuwonjezera madzi kuti mutsimikizire kusakaniza bwino. Njira yosungiramo iyenera kutsukidwa ndikusungidwa mufiriji kuti musamawume mobwerezabwereza. Sungunulani njira yosungiramo ndi chikhalidwe cha chikhalidwe mpaka ndende yofunikira, ndipo konzekerani njira yogwirira ntchito pomwepo ndikuigwiritsa ntchito nthawi yomweyo.

Pomaliza, zeatin, abscisic acid ndi abscisic acid nucleotide aliyense ali ndi makhalidwe ake malinga ndi kapangidwe, ntchito ndi ntchito ntchito. Komabe, zonse zimagwira ntchito ngati zowongolera kukula kwa mbewu ndipo zimathandizira kwambiri kulimbikitsa kukula ndi kukula kwa mbewu.