Ntchito zazikulu
1. Kulimbikitsa kugawa kwa maselo, makamaka kugawa kwa cytoplasm;
2. Kulimbikitsa kusiyana kwa mphukira. Pakukula kwa minofu, imagwirizana ndi auxin kuti ilamulire kusiyana ndi kupangika kwa mizu ndi mphukira;
3. Kulimbikitsa kukula kwa mphukira za mbali, kuthetsa kulamulira kwa nthambi, motero kumabweretsa kupangika kwa mphukira zambiri zomwe zimamera m'thupi;
4. Kuchedwetsa kukalamba kwa masamba, kuchepetsa kuchepa kwa chlorophyll ndi mapuloteni;
5. Dulani mbewu kuti zisaume, sinthani kuwala kuti zikwaniritse zofunikira pa kuwala kwa mbewu monga fodya;
6. Kuyambitsa parthenocarpy mu zipatso zina;
7. Kulimbikitsa kupangika kwa zilembo zoyambira za mphukira: kumapeto kwa masamba ndi m'malo ena a mosses, zimatha kulimbikitsa kupangika kwa zilembo zoyambira za mphukira;
8. Limbikitsani kupangika kwa mizu ya mbatata.
Ili ndi kapangidwe ka trans kokha ndipo ili ndi zotsatira zofanana ndizeatin, koma ndi ntchito yolimba.
Mphamvu yake ndi yofanana kwambiri ndi ya anti-zeatin. Sikuti imangokhala ndi ntchito za zeatin zomwe zatchulidwa pamwambapa, komanso imagwiranso ntchito yoyambitsa kufotokozera majini ndi kagayidwe kachakudya.
Njira Yogwiritsira Ntchito
1. Kulimbikitsa kumera kwa callus (kuyenera kugwiritsidwa ntchito limodzi ndi auxin), kuchuluka kwa 1mg/L.
2. Limbikitsani kuyika zipatso, 1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, thirani zipatso patatha masiku 10, 25, ndi 40 mutatulutsa maluwa.
3. Pa ndiwo zamasamba, thirani pa 201 mg/L kuti tsamba lichedwetse chikasu.
Kuphatikiza apo, kuchiza mbewu zina za mbewu kungathandize kumera; kuchiza mbande pa nthawi yobzala kungathandize kukula.
1. Kulimbikitsa kumera kwa minofu ya callus (iyenera kugwiritsidwa ntchito limodzi ndi auxin), pamlingo wa 1 ppm;
2. Limbikitsani kuyika zipatso, 100 ppm ya cytokinin + 500 ppm ya GA3 + 20 ppm ya NAA, poperani zipatsozo masiku 10, 25, ndi 40 mutatulutsa maluwa;
3. Chepetsani chikasu cha masamba a masamba, thirani 20 ppm;
1. Mu ulimi wa minofu ya zomera, kuchuluka kwa anti-cytokinin nucleoside komwe kumafala kwambiri ndi 1 mg/mL kapena kupitirira apo.
2. Mu malamulo okhwima a kukula kwa zomera, kuchuluka kwa anti-cytokinin nucleoside nthawi zambiri kumakhala pakati pa 1 ppm mpaka 100 ppm, ndipo kuchuluka kwake kumadalira momwe zimagwiritsidwira ntchito komanso mtundu wa zomera. Mwachitsanzo, polimbikitsa kumera kwa minofu ya callus, kuchuluka kwa anti-cytokinin nucleoside ndi 1 ppm, ndipo imafunika kugwiritsidwa ntchito limodzi ndi auxin.
3. Sungunulani ufa wa anti-cytokinin nucleoside bwino ndi 2-5 mL ya 1 M NaOH (kapena 1 M acetic acid kapena 1 M KOH), kenako onjezerani madzi osungunuka kawiri kapena madzi oyera kwambiri kuti mukonze yankho losungira la 1 mg/mL kapena kuchuluka kwakukulu. Sakanizani mukuwonjezera madzi kuti muwonetsetse kuti mukusakaniza bwino. Njira yosungira iyenera kugawidwa ndikusungidwa mufiriji kuti mupewe kuzizira mobwerezabwereza. Sakanizani njira yosungira ndi njira yosungiramo zinthu kuti ikhale yokwanira, ndipo konzani njira yogwirira ntchito pamalopo ndikugwiritsa ntchito nthawi yomweyo.
Pomaliza, zeatin, abscisic acid ndi abscisic acid nucleotide iliyonse ili ndi makhalidwe akeake pankhani ya kapangidwe kake, ntchito yake komanso momwe imagwirira ntchito. Pomaliza, zeatin, abscisic acid ndi abscisic acid nucleotide iliyonse ili ndi makhalidwe akeake pankhani ya kapangidwe kake, ntchito yake komanso momwe imagwirira ntchito. Komabe, zonsezi zimagwira ntchito ngati owongolera kukula kwa zomera ndipo zimagwira ntchito yofunika kwambiri pakulimbikitsa kukula ndi chitukuko cha zomera.
Nthawi yotumizira: Okutobala-22-2025