Chowongolera kukula kwa phwetekere cha 5-aminolevulinic acid chimawonjezera kukana kuzizira kwa zomera.

      Monga chimodzi mwa zinthu zazikulu zomwe zimapangitsa kuti zomera zisavutike, kutentha kochepa kumalepheretsa kukula kwa zomera ndipo kumakhudza kwambiri zokolola ndi ubwino wa mbewu. 5-Aminolevulinic acid (ALA) ndi chinthu chowongolera kukula chomwe chimapezeka kwambiri m'zinyama ndi zomera. Chifukwa cha mphamvu zake zapamwamba, zopanda poizoni komanso zosavuta kuziwononga, chimagwiritsidwa ntchito kwambiri poletsa kuzizira kwa zomera.
Komabe, kafukufuku wambiri waposachedwa wokhudzana ndi ALA makamaka amayang'ana kwambiri pakulamulira maukonde. Kachitidwe ka mamolekyulu a ALA polimbana ndi kuzizira kwa zomera sikudziwika bwino pakadali pano ndipo asayansi amafunikira kafukufuku wowonjezereka.
Mu Januwale 2024, Horticultural Research inafalitsa pepala lofufuza lotchedwa “5-Aminolevulinic Acid Imawonjezera Kulekerera Kuzizira Mwa Kulamulira SlMYB4/SlMYB88-SlGSTU43 Reactive Oxygen Species Scavenging Module mu Tomato” lolembedwa ndi gulu la Hu Xiaohui ku Northwestern University agriculture and forestry.
Mu kafukufukuyu, jini ya glutathione S-transferase SlGSTU43 idapezeka mu phwetekere (Solanum lycopersicum L.). Zotsatira za kafukufukuyu zidawonetsa kuti ALA imayambitsa kwambiri SlGSTU43 pamene ikuzizira. Mizere ya phwetekere yosinthika ya SlGSTU43 idawonetsa kuchuluka kwa mphamvu yolandirira mpweya wa okosijeni ndipo idawonetsa kukana kwakukulu ku kutentha kochepa, pomwe mizere ya SlGSTU43 yosinthika inali yodziwika bwino ndi kutentha kochepa.
Kuphatikiza apo, zotsatira za kafukufukuyu zasonyeza kuti ALA siikulitsa kupirira kwa mtundu wa mutant mpaka kutentha kochepa. Chifukwa chake, kafukufukuyu akusonyeza kuti SlGSTU43 ndi jini yofunika kwambiri pakukulitsa kupirira kuzizira mu phwetekere ndi ALA (Chithunzi 1).
Kuphatikiza apo, kafukufukuyu adatsimikizira kudzera mu EMSA, Y1H, LUC ndi ChIP-qPCR kuti SlMYB4 ndi SlMYB88 zimatha kuwongolera momwe SlGSTU43 imagwirira ntchito polumikizana ndi cholimbikitsira cha SlGSTU43. Kuyesera kwina kunawonetsa kuti SlMYB4 ndi SlMYB88 zimakhudzidwanso ndi njira ya ALC powonjezera kupirira kwa phwetekere ku kutentha kochepa komanso kuwongolera momwe SlGSTU43 imagwirira ntchito (Chithunzi 2). Zotsatirazi zimapereka chidziwitso chatsopano pa njira yomwe ALA imathandizira kupirira ku kutentha kochepa mu phwetekere.
Zambiri: Zhengda Zhang et al., 5-aminolevulinic acid imawonjezera kupirira kuzizira mwa kulamulira gawo la SlMYB4/SlMYB88-SlGSTU43 la mitundu ya okosijeni yolandirira mu phwetekere, Horticulture Research (2024). DOI: 10.1093/hour/uhae026
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