Ntchito ya Uniconazole

       Uniconazolendi triazolechowongolera kukula kwa zomerazomwe zimagwiritsidwa ntchito kwambiri polamulira kutalika kwa chomera ndikuletsa kukula kwa mbande. Komabe, njira ya molekyulu yomwe uniconazole imaletsera kutalika kwa mbande ya hypocotyl sikudziwikabe, ndipo pali maphunziro ochepa okha omwe amaphatikiza deta ya transcriptome ndi metabolome kuti afufuze momwe hypocotyl elongation imagwirira ntchito. Pano, tawona kuti uniconazole idaletsa kwambiri kutalika kwa hypocotyl mu mbande za kabichi zamaluwa aku China. Chosangalatsa n'chakuti, kutengera kusanthula kophatikizana kwa transcriptome ndi metabolome, tapeza kuti uniconazole idakhudza kwambiri njira ya "phenylpropanoid biosynthesis". Munjira iyi, jini imodzi yokha ya banja la majini olamulira enzyme, BrPAL4, yomwe imagwira ntchito mu lignin biosynthesis, idachepetsedwa kwambiri. Kuphatikiza apo, kuyesa kwa yisiti imodzi yosakanikirana ndi iwiri kwawonetsa kuti BrbZIP39 ikhoza kumangirira mwachindunji kudera lolimbikitsira la BrPAL4 ndikuyambitsa kulembedwa kwake. Dongosolo loletsa majini lomwe limayambitsidwa ndi kachilomboka linatsimikiziranso kuti BrbZIP39 ikhoza kuwongolera bwino kutalika kwa kabichi waku China ndi kapangidwe ka hypocotyl lignin. Zotsatira za kafukufukuyu zikupereka chidziwitso chatsopano pa njira yowongolera mamolekyu a cloconazole poletsa kutalika kwa kabichi waku China. Zatsimikiziridwa koyamba kuti cloconazole imachepetsa kuchuluka kwa lignin poletsa kupanga phenylpropanoid komwe kumayendetsedwa ndi gawo la BrbZIP39-BrPAL4, motero zimapangitsa kuti mbande za kabichi waku China zikhale zochepa.

Kabichi waku China (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) ndi wa mtundu wa Brassica ndipo ndi ndiwo zamasamba zodziwika bwino zomwe zimamera kwambiri chaka chilichonse m'dziko langa (Wang et al., 2022; Yue et al., 2022). M'zaka zaposachedwa, kuchuluka kwa kolifulawa waku China kwapitirira kukula, ndipo njira yolima yasintha kuchoka pa kubzala mwachindunji kupita ku kubzala mbewu mwachangu komanso kubzala mbande. Komabe, pakubzala mbande mwachangu komanso kubzala mbande, kukula kwambiri kwa hypocotyl kumabweretsa mbande zoyenda, zomwe zimapangitsa kuti mbande zisakule bwino. Chifukwa chake, kuwongolera kukula kwambiri kwa hypocotyl ndi vuto lalikulu pakubzala mbande mwachangu komanso kubzala kabichi waku China. Pakadali pano, pali maphunziro ochepa omwe akuphatikiza deta ya transcriptomics ndi metabolomics kuti afufuze momwe hypocotyl elongation imagwirira ntchito. Njira ya mamolekyu yomwe chlorantazole imawongolera kukula kwa hypocotyl mu kabichi waku China sinaphunziridwebe. Cholinga chathu chinali kupeza majini ndi njira zamamolekyu zomwe zimayankha ku hypocotyl dwarfing yomwe imachitika chifukwa cha uniconazole mu kabichi waku China. Pogwiritsa ntchito kusanthula kwa transcriptome ndi metabolomic, komanso kusanthula kwa yeast one-hybrid, dual luciferase assay, ndi virus-induced gene silencing (VIGS) assay, tinapeza kuti uniconazole ikhoza kuyambitsa hypocotyl dwarfing mu kabichi waku China poletsa lignin biosynthesis mu mbande za kabichi waku China. Zotsatira zathu zimapereka chidziwitso chatsopano pa njira yowongolera mamolekyu yomwe uniconazole imaletsa hypocotyl elongation mu kabichi waku China poletsa phenylpropanoid biosynthesis yomwe imayendetsedwa ndi gawo la BrbZIP39–BrPAL4. Zotsatirazi zitha kukhala ndi tanthauzo lofunikira pakukweza ubwino wa mbande zamalonda ndikuthandizira kuonetsetsa kuti ndiwo zamasamba zikukolola bwino komanso kukhala ndi ubwino.
BrbZIP39 ORF yonse inalowetsedwa mu pGreenll 62-SK kuti ipange effector, ndipo chidutswa cha promoter cha BrPAL4 chinaphatikizidwa ku jini ya pGreenll 0800 luciferase (LUC) reporter kuti ipange jini ya reporter. Ma vector a effector ndi reporter gene anasinthidwa kukhala masamba a fodya (Nicotiana benthamiana).
Kuti timvetse bwino ubale wa metabolites ndi majini, tinachita kusanthula kwa metabolome ndi transcriptome yolumikizana. Kusanthula kwa KEGG pathway enrichment kunawonetsa kuti ma DEG ndi ma DAM adalumikizidwa pamodzi m'njira 33 za KEGG (Chithunzi 5A). Pakati pawo, njira ya "phenylpropanoid biosynthesis" inali yolemera kwambiri; njira ya "photosynthetic carbon fixation", njira ya "flavonoid biosynthesis", njira ya "pentose-glucuronic acid interconversion", njira ya "tryptophan metabolism", ndi njira ya "starch-sucrose metabolism" zinawonjezekanso kwambiri. Mapu osonkhanitsa kutentha (Chithunzi 5B) adawonetsa kuti ma DAM ogwirizana ndi ma DEG adagawidwa m'magulu angapo, omwe ma flavonoid anali gulu lalikulu kwambiri, zomwe zikusonyeza kuti njira ya "phenylpropanoid biosynthesis" idasewera gawo lofunikira kwambiri mu hypocotyl dwarfism.
Olembawo anena kuti kafukufukuyu anachitika popanda ubale uliwonse wamalonda kapena zachuma womwe ungatanthauzidwe ngati mkangano wokhudzana ndi chidwi.
Malingaliro onse omwe aperekedwa munkhaniyi ndi a wolemba yekha ndipo sakusonyeza maganizo a mabungwe ogwirizana nawo, ofalitsa, akonzi, kapena owunikira. Zinthu zilizonse zomwe zawunikidwa munkhaniyi kapena zomwe opanga ake amanena sizikutsimikiziridwa kapena kuvomerezedwa ndi wofalitsa.