Uniconazole ntchito

       Uniconazolendi triazolechowongolera kukula kwa mbewuzomwe zimagwiritsidwa ntchito kwambiri poyang'anira kutalika kwa mbewu ndikuletsa kukula kwa mbande. Komabe, njira ya maselo yomwe uniconazole imalepheretsa mbande kukweza kwa hypocotyl sikudziwikabe, ndipo pali maphunziro ochepa omwe amaphatikiza deta ya transcriptome ndi metabolome kuti afufuze momwe hypocotyl elongation imagwirira ntchito. Apa, tawona kuti uniconazole kwambiri inhibited hypocotyl elongation mu Chinese maluwa kabichi mbande. Chochititsa chidwi n'chakuti, potengera kusanthula kwa transcriptome ndi metabolome, tinapeza kuti uniconazole inakhudza kwambiri njira ya "phenylpropanoid biosynthesis". Mwanjira iyi, jini imodzi yokha ya banja loyang'anira ma enzyme, BrPAL4, yomwe imakhudzidwa ndi lignin biosynthesis, idachepetsedwa kwambiri. Kuphatikiza apo, kuyesa kwa yisiti kwa haibridi imodzi ndi mitundu iwiri yosakanizidwa yawonetsa kuti BrbZIP39 ikhoza kumangirira mwachindunji kudera la BrPAL4 ndikuyambitsa kumasulira kwake. Dongosolo loletsa kuletsa jini loyambitsidwa ndi ma virus linatsimikiziranso kuti BrbZIP39 imatha kuwongolera kutalika kwa kabichi waku China ndi kaphatikizidwe ka hypocotyl lignin. Zotsatira za kafukufukuyu zikupereka zidziwitso zatsopano za momwe cloconazole imagwirira ntchito poletsa kukula kwa hypocotyl kwa kabichi waku China. Zinatsimikiziridwa kwa nthawi yoyamba kuti cloconazole imachepetsa kuchuluka kwa lignin poletsa kaphatikizidwe ka phenylpropanoid mothandizidwa ndi gawo la BrbZIP39-BrPAL4, zomwe zimapangitsa kuti mbande za kabichi za ku China zikhale zochepa kwambiri.

Kabichi waku China (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) ndi wamtundu wa Brassica ndipo ndi ndiwo zamasamba zodziwika bwino zapachaka zomwe zimabzalidwa kwambiri mdziko lathu (Wang et al., 2022; Yue et al., 2022). M'zaka zaposachedwa, kukula kwa kolifulawa waku China kwapitilira kukula, ndipo njira yolima yasintha kuchoka pachikhalidwe chachindunji kupita ku chikhalidwe chamba komanso kuyika. Komabe, pakukula kwambiri kwa mbande ndikuyika, kukula kwakukulu kwa hypocotyl kumatulutsa mbande zamiyendo, zomwe zimapangitsa kuti mbande ikhale yabwino. Chifukwa chake, kuwongolera kukula kwa hypocotyl ndizovuta kwambiri pachikhalidwe chambande komanso kuyika kabichi waku China. Pakadali pano, pali maphunziro ochepa omwe amaphatikiza ma transcriptomics ndi metabolomics kuti afufuze momwe ma hypocotyl elongation amagwirira ntchito. Makina a maselo omwe chlorantazole amawongolera kukula kwa hypocotyl mu kabichi waku China sanaphunzirepo. Tidafuna kudziwa kuti ndi ma jini ndi mamolekyu ati omwe amayankha ku uniconazole-induced hypocotyl dwarfing mu kabichi waku China. Pogwiritsa ntchito kusanthula kwa transcriptome ndi metabolomic, komanso kusanthula kwa yisiti imodzi-hybrid, kuyesa kwapawiri kwa luciferase, ndi kuyesa kwa gene kuletsa ma virus (VIGS), tapeza kuti uniconazole imatha kuyambitsa hypocotyl dwarfing mu kabichi waku China poletsa lignin biosynthesis mu mbewu za kabichi zaku China. Zotsatira zathu zimapereka zidziwitso zatsopano zamakina owongolera ma cell omwe uniconazole amalepheretsa kutalika kwa hypocotyl mu kabichi waku China kudzera mu kuletsa phenylpropanoid biosynthesis yolumikizidwa ndi gawo la BrbZIP39-BrPAL4. Zotsatirazi zitha kukhala ndi tanthauzo lofunikira pakukweza mbande zamalonda ndikuwonetsetsa kuti zokolola zamasamba ndi zabwino.
BrbZIP39 ORF yautali wonse idayikidwa mu pGreenll 62-SK kuti ipange chochita, ndipo kachigawo kolimbikitsa ka BrPAL4 idaphatikizidwa ku jini ya mtolankhani wa pGreenll 0800 luciferase (LUC) kuti apange jini ya mtolankhani. The effector and reporter gene vectors were co-transformed to Fodya (Nicotiana benthamiana) masamba.
Kuti timveketse maubwenzi a metabolites ndi majini, tidachita kusanthula kwa metabolome ndi transcriptome. Kusanthula kwa njira za KEGG kunawonetsa kuti ma DEG ndi ma DAM adalemeretsedwa munjira za 33 KEGG (Chithunzi 5A). Pakati pawo, njira ya "phenylpropanoid biosynthesis" inali yolemetsedwa kwambiri; njira ya "photosynthetic carbon fixation", "flavonoid biosynthesis" njira, njira ya "pentose-glucuronic acid interconversion", njira ya "tryptophan metabolism", ndi "starch-sucrose metabolism" nayonso inalemeretsedwa kwambiri. Mapu opangira kutentha (Chithunzi 5B) adawonetsa kuti ma DAM ogwirizana ndi DEGs adagawidwa m'magulu angapo, omwe flavonoids anali gulu lalikulu kwambiri, zomwe zimasonyeza kuti njira ya "phenylpropanoid biosynthesis" inathandiza kwambiri mu hypocotyl dwarfism.
Olembawo akulengeza kuti kafukufukuyu adachitidwa popanda maubwenzi amalonda kapena azachuma omwe angatanthauzidwe ngati kusagwirizana kwa chidwi.
Malingaliro onse omwe afotokozedwa m'nkhaniyi ndi a wolemba okha ndipo samasonyeza maganizo a mabungwe ogwirizana, osindikiza, olemba, kapena owunikira. Zogulitsa zilizonse zomwe zawunikidwa m'nkhaniyi kapena zonenedweratu ndi opanga sizikutsimikiziridwa kapena kuvomerezedwa ndi wosindikiza.