Ofufuza apeza njira yoyendetsera mapuloteni a DELLA muzomera.

Ofufuza ochokera ku dipatimenti ya Biochemistry ku Indian Institute of Sciences (IISc) apeza njira yomwe yakhala ikufunidwa kwa nthawi yayitali yomwe imagwiritsidwa ntchito ndi zomera zakale monga bryophytes (kuphatikiza mosses ndi chiwindi)kuwongolera kukula kwa mbewu- njira yomwe yasungidwanso muzomera zamaluwa zomwe zasinthika posachedwa.

Kafukufukuyu, wofalitsidwa mu nyuzipepala ya Nature Chemical Biology, akuyang'ana kwambiri malamulo osakhala akale a mapuloteni a DELLA, owongolera kukula omwe amatha kuletsa kugawanika kwa maselo muzomera za embryonic (zomera zapamtunda).
Debabrata Laha, pulofesa wothandizira wa biochemistry komanso wolemba nawo kafukufukuyu akufotokoza kuti: "DELLA imachita ngati kugunda kwa liwiro, koma ngati kugunda kwa liwiro kumakhalapo nthawi zonse, mbewuyo simatha kusuntha. Chifukwa chake, kuwonongeka kwa mapuloteni a DELLA ndikofunikira kwambiri pakulimbikitsa kukula kwa mbewu. Muzomera zamaluwa, DELLA imawonongeka pamene phytohormonegibberellin (GA)imamangiriza ku cholandilira chake GID1, kupanga chovuta cha GA-GID1-DELLA. Pambuyo pake, mapuloteni opondereza a DELLA amamangirira ku ubiquitin unyolo ndipo amanyozedwa ndi 26S proteasome.
Chochititsa chidwi n'chakuti, ma bryophyte anali m'gulu la zomera zoyamba kulamulira nthaka, pafupifupi zaka 500 miliyoni zapitazo. Ngakhale amatulutsa phytohormone gibberellin (GA), alibe cholandilira cha GID1. Izi zimadzutsa funso lakuti: Kodi kakulidwe ndi kakulidwe ka zomera zoyamba zapamtunda zimenezi zinayendetsedwa bwanji?
Ofufuzawa adagwiritsa ntchito dongosolo la CRISPR-Cas9 kugwetsa jini yofananira ya VIH, potero kutsimikizira udindo wa VIH. Zomera zomwe zilibe puloteni ya VIH yogwira ntchito zimawonetsa kukula kwakukulu ndi zolakwika zachitukuko ndi zolakwika za morphological, monga wandiweyani wa thallus, kukula kwa radial, ndi kusowa kwa calyx. Zolakwika izi zidakonzedwa posintha genome ya mbewu kuti ipange gawo limodzi lokha (N-terminus) ya enzyme ya VIH. Pogwiritsa ntchito njira zapamwamba za chromatography, gulu lofufuza lidapeza kuti N-terminus ili ndi domain kinase yomwe imathandizira kupanga InsP₈.
Ofufuzawa adapeza kuti DELLA ndi imodzi mwazomwe zimayendera ma cell a VIH kinase. Komanso, adawona kuti phenotype ya zomera zoperewera za MpVIH zinali zofanana ndi za Miscanthus multiforme zomera zokhala ndi DELLA yowonjezera.
"Pakadali pano, tikufunitsitsa kudziwa ngati kukhazikika kwa DELLA kapena ntchito zikuchulukirachulukira muzomera zopanda MpVIH," atero a Priyanshi Rana, wophunzira udokotala mu gulu lofufuza la Lahey komanso wolemba woyamba wa pepalalo. Mogwirizana ndi malingaliro awo, ofufuzawo adapeza kuti kuletsa kwa DELLA kunabwezeretsanso kukula ndi zovuta zakukula kwa mbewu zosinthika za MpVIH. Zotsatirazi zikusonyeza kuti VIH kinase imayendetsa molakwika DELLA, motero imalimbikitsa kukula ndi kukula kwa zomera.
Ofufuzawa adaphatikiza njira zama genetic, biochemical, ndi biophysical kuti afotokozere momwe inositol pyrophosphate imawongolera mawonekedwe a mapuloteni a DELLA mu bryophyte iyi. Makamaka, InsP₈, yopangidwa ndi MpVIH, imamangiriza ku mapuloteni a MpDELLA, kulimbikitsa polyubiquitination, zomwe zimapangitsa kuwonongeka kwa mapuloteni opondereza ndi proteasome.
Kafukufuku wokhudza puloteni ya DELLA adayambira pa Green Revolution, pomwe asayansi mosadziwa adagwiritsa ntchito mwayi wake kuti apange mitundu yokolola kwambiri yamitundu yocheperako. Ngakhale kuti kachitidwe kake sikunali kodziwika panthawiyo, matekinoloje amakono athandiza asayansi kugwiritsa ntchito kusintha kwa majini kuti awononge ntchito ya mapuloteniwa, potero akuwonjezera zokolola za mbewu.
"Chifukwa cha kukwera kwa chiwerengero cha anthu komanso kuchepa kwa malo olima, kuchulukitsa zokolola kwakhala kovuta," adatero Raha. Poganizira kuti kuwonongeka kwa InsP₈-regulated DELLA kumatha kufalikira m'zomera za embryonic, zomwe zapezekazi zitha kutsegulira njira yopangira mbewu zokolola kwambiri za m'badwo wotsatira.