Ofufuza apeza momwe zomera zimayendera mapuloteni a DELLA.

Ofufuza ochokera ku dipatimenti ya Biochemistry ku Indian Institute of Sciences (IISc) apeza njira yomwe anthu akhala akufuna kwanthawi yayitalipofuna kuyang'anira kukula kwa zomera zakale zapamtunda monga bryophytes (gulu lomwe limaphatikizapo mosses ndi liworts) zomwe zimasungidwa muzomera zamaluwa zamtsogolo.
Kafukufukuyu, wofalitsidwa mu nyuzipepala ya Nature Chemical Biology, adayang'ana kwambiri pakuwongolera kosavomerezeka kwa mapuloteni a DELLA, wowongolera kukula omwe amalepheretsa kugawanika kwa ma cell mu embryophytes (zomera zakumtunda).
Chosangalatsa ndichakuti, bryophytes, mbewu zoyamba kuwoneka pamtunda zaka pafupifupi 500 miliyoni zapitazo, zilibe cholandilira cha GID1, ngakhale zimapanga phytohormone GA. Izi zimadzutsa funso la momwe kakulidwe ndi kakulidwe ka zomera zoyamba zapamtundazi zimayendetsedwa.
Pogwiritsa ntchito liverwort Marchantia polymorpha monga chitsanzo, ofufuza adapeza kuti zomera zakalezi zimagwiritsa ntchito puloteni yapadera, MpVIH, yomwe imapanga messenger ya cell inositol pyrophosphate (InsP₈), kuti iwononge DELLA popanda kutenga nawo mbali.gibberellik asidi.
Ofufuzawa adagwiritsa ntchito dongosolo la CRISPR-Cas9 kuti agwetse jini yomwe imayika enzyme ya VIH, kutsimikizira ntchito yake. Zomera zosagwira ntchito VIH zimawonetsa zolakwika zazikulu zachitukuko ndi zolakwika za morphological, monga masamba ophatikizika, kukula kwa radial, ndi kusowa kwa ma calyxes. Zolakwika izi zidathetsedwa posintha genome ya mbewu kuti ipange malekezero amodzi okha (N-terminus) ya enzyme ya VIH. Pogwiritsa ntchito njira zapamwamba za chromatography, gululo lidazindikira kuti N-terminus ili ndi domain kinase yomwe imathandizira kupanga InsP₈.
Ofufuzawa adapeza kuti DELLA ndi imodzi mwazomwe zimayendera ma cell a VIH kinase. Komanso, adawona kuti ma phenotypes a zomera zoperewera za MpVIH anali ofanana ndi a zomera za M. polymorpha zokhala ndi mawu owonjezereka a DELLA.
"Pakadali pano, tinali okondwa kumvetsetsa ngati kukhazikika kwa DELLA kapena ntchito zikuchulukirachulukira muzomera zomwe zili ndi MpVIH," atero Priyanshi Rana, wolemba woyamba komanso wophunzira womaliza pagulu lofufuza la Lahey. Mogwirizana ndi malingaliro awo, ofufuzawo adapeza kuti kuletsa kwa DELLA kumatha kubwezeretsanso kukula kolakwika ndi chitukuko chazomera za MpVIH zosinthika. Zotsatirazi zikusonyeza kuti VIH kinase imayendetsa molakwika DELLA, kulimbikitsa kukula ndi chitukuko cha zomera.
Kafukufuku wokhudza mapuloteni a DELLA adayambira pa Green Revolution, pomwe asayansi mosadziwa adagwiritsa ntchito mwayi wawo kuti apange mitundu yochuluka yokolola yocheperako. Ngakhale kuti tsatanetsatane wa ntchito yawo sankadziwika panthawiyo, luso lamakono limalola asayansi kusokoneza ntchito za mapuloteniwa pogwiritsa ntchito majini, ndikuwonjezera zokolola za mbewu.