Ofufuza apeza momwe zomera zimayendetsera mapuloteni a DELLA.

Ofufuza ochokera ku Dipatimenti ya Biochemistry ku Indian Institute of Sciences (IISc) apeza njira yomwe yakhala ikufunidwa kwa nthawi yayitalipoyang'anira kukula kwa zomera zakale monga bryophytes (gulu lomwe limaphatikizapo mosses ndi liverworts) zomwe zidasungidwa m'zomera zomwe zidatulutsa maluwa pambuyo pake.
Kafukufukuyu, wofalitsidwa mu magazini ya Nature Chemical Biology, adayang'ana kwambiri pa malamulo osavomerezeka a mapuloteni a DELLA, omwe ndi olamulira wamkulu wa kukula kwa maselo omwe amaletsa kugawikana kwa maselo m'ma embryophytes (zomera zakuthengo).
Chochititsa chidwi n'chakuti, bryophytes, zomera zoyamba kuwonekera panthaka pafupifupi zaka 500 miliyoni zapitazo, sizili ndi cholandirira cha GID1, ngakhale kuti zimapanga phytohormone GA. Izi zikubweretsa funso la momwe kukula ndi chitukuko cha zomera zoyambirira zapanthaka izi zinalamuliridwira.
Pogwiritsa ntchito liverwort Marchantia polymorpha ngati njira yotsanzira, ofufuzawa adapeza kuti zomera zakalezi zimagwiritsa ntchito enzyme yapadera, MpVIH, yomwe imapanga cell messenger inositol pyrophosphate (InsP₈), kuti iwononge DELLA popanda kulowererapo kwaasidi wa gibberellic.
Ofufuzawa adagwiritsa ntchito njira ya CRISPR-Cas9 kuti athetse jini yomwe imapanga enzyme ya VIH, kutsimikizira ntchito yake. Zomera zopanda VIH yogwira ntchito zinawonetsa zolakwika zazikulu pakukula ndi zolakwika za kapangidwe kake, monga masamba opapatiza, kukula kolakwika kwa radial, komanso kusowa kwa calyxes. Zolakwika izi zidachotsedwa posintha genome ya chomera kuti ipange mbali imodzi yokha (N-terminus) ya enzyme ya VIH. Pogwiritsa ntchito njira zapamwamba za chromatography, gululi linapeza kuti N-terminus ili ndi gawo la kinase lomwe limayambitsa kupanga InsP₈.
Ofufuzawo adapeza kuti DELLA ndi imodzi mwa zolinga za maselo a VIH kinase. Kuphatikiza apo, adawona kuti mawonekedwe a zomera zopanda MpVIH anali ofanana ndi a zomera za M. polymorpha zomwe zimakhala ndi mawonekedwe owonjezereka a DELLA.
"Panthawiyi, tinali okondwa kumvetsetsa ngati kukhazikika kwa DELLA kapena ntchito zake zikuwonjezeka m'zomera zopanda MpVIH," anatero Priyanshi Rana, wolemba woyamba komanso wophunzira womaliza maphunziro mu gulu lofufuza la Lahey. Mogwirizana ndi lingaliro lawo, ofufuzawo adapeza kuti kuletsa kwa DELLA kumatha kubwezeretsa kwambiri mawonekedwe olakwika a kukula ndi chitukuko cha zomera zosinthika za MpVIH. Zotsatirazi zikusonyeza kuti VIH kinase imalamulira molakwika DELLA, ndikulimbikitsa kukula ndi chitukuko cha zomera.
Kafukufuku wa mapuloteni a DELLA unayamba kalekale pa Green Revolution, pomwe asayansi mosadziwa adagwiritsa ntchito mphamvu zawo popanga mitundu ya semi-dwarf yobala zipatso zambiri. Ngakhale kuti tsatanetsatane wa ntchito yawo sunali womveka bwino panthawiyo, ukadaulo wamakono umalola asayansi kusintha magwiridwe antchito a mapuloteniwa kudzera muukadaulo wa majini, ndikuwonjezera zokolola za mbewu.