Paclobutrazol imayambitsa kupanga kwa triterpenoid mwa kuletsa negative transcriptional regulator SlMYB mu honeysuckle yaku Japan.

Bowa lalikulu lili ndi zinthu zambiri zosiyanasiyana zomwe zimagwira ntchito m'thupi ndipo limaonedwa kuti ndi zinthu zofunika kwambiri m'thupi. Phellinus igniarius ndi bowa lalikulu lomwe nthawi zambiri limagwiritsidwa ntchito pochiza komanso pochiza matenda, koma kugawidwa kwake ndi dzina lake lachilatini kukupitirirabe kutsutsana. Pogwiritsa ntchito kusanthula kwa magawo ambiri a majini, ofufuzawo adatsimikiza kuti Phellinus igniarius ndi mitundu yofanana ndi ya mtundu watsopano ndipo adakhazikitsa mtundu wa Sanghuangporus. Bowa wa honeysuckle Sanghuangporus lonicericola ndi umodzi mwa mitundu ya Sanghuangporus yomwe yadziwika padziko lonse lapansi. Phellinus igniarius yakopa chidwi chachikulu chifukwa cha mankhwala ake osiyanasiyana, kuphatikizapo ma polysaccharides, ma polyphenols, ma terpenes, ndi ma flavonoids. Ma Triterpenes ndi mankhwala ofunikira kwambiri omwe amagwira ntchito m'thupi la mtundu uwu, omwe amasonyeza antioxidant, antibacterial, komanso antitumor.
Ma Triterpenoid ali ndi kuthekera kwakukulu kogwiritsidwa ntchito m'mabizinesi. Chifukwa cha kusowa kwa zinthu zakuthengo za Sanghuangporus m'chilengedwe, kukulitsa bwino ntchito yake yopanga ndi kukolola ndikofunikira kwambiri. Pakadali pano, kupita patsogolo kwachitika pakukweza kupanga ma metabolites osiyanasiyana achiwiri a Sanghuangporus pogwiritsa ntchito mankhwala oyambitsa kuipitsa madzi. Mwachitsanzo, ma polyunsaturated fatty acids, fungal elicitors11 ndi phytohormones (kuphatikiza methyl jasmonate ndi salicylic acid14) zawonetsedwa kuti zikuwonjezera kupanga kwa triterpenoid mu Sanghuangporus. Oyang'anira kukula kwa zomera(Ma PGR)akhoza kuwongolera kapangidwe ka zinthu zina zomwe zimapezeka m'zomera. Mu kafukufukuyu, PBZ, chowongolera kukula kwa zomera chomwe chimagwiritsidwa ntchito kwambiri kuwongolera kukula kwa zomera, kukolola, ubwino ndi makhalidwe a thupi, chinafufuzidwa. Makamaka, kugwiritsa ntchito PBZ kungakhudze njira ya terpenoid biosynthetic m'zomera. Kuphatikiza kwa gibberellins ndi PBZ kunawonjezera kuchuluka kwa quinone methide triterpene (QT) mu Montevidia floribunda. Kapangidwe ka njira ya terpenoid ya mafuta a lavender kanasinthidwa pambuyo pothandizidwa ndi 400 ppm PBZ. Komabe, palibe malipoti okhudza kugwiritsa ntchito PBZ ku bowa.
Kuwonjezera pa maphunziro omwe akuyang'ana kwambiri kuwonjezeka kwa kupanga triterpene, maphunziro ena afotokozanso njira zowongolera za triterpene biosynthesis mu Moriformis motsogozedwa ndi mankhwala oyambitsa mankhwala. Pakadali pano, maphunziro akuyang'ana kwambiri kusintha kwa kuchuluka kwa majini opangidwa okhudzana ndi triterpene biosynthesis mu njira ya MVA, zomwe zimapangitsa kuti pakhale kuwonjezeka kwa kupanga terpenoid.12,14 Komabe, njira zomwe zimayambira majini odziwika awa, makamaka zinthu zolembera zomwe zimawongolera mawonekedwe awo, sizikudziwika bwino m'njira zowongolera za triterpene biosynthesis mu Moriformis.
Mu kafukufukuyu, zotsatira za kuchuluka kosiyanasiyana kwa owongolera kukula kwa zomera (PGRs) pa kupanga triterpene ndi kukula kwa mycelial panthawi yophika honeysuckle (S. lonicericola) pansi pa madzi zinafufuzidwa. Pambuyo pake, metabolomics ndi transcriptomics zinagwiritsidwa ntchito pofufuza kapangidwe ka triterpene ndi machitidwe a majini omwe amagwiritsidwa ntchito mu triterpene biosynthesis panthawi ya chithandizo cha PBZ. Deta ya RNA-sequencing ndi bioinformatics inapezanso target transcription factor ya MYB (SlMYB). Kuphatikiza apo, ma mutants adapangidwa kuti atsimikizire mphamvu yolamulira ya jini ya SlMYB pa triterpene biosynthesis ndikuzindikira majini omwe angakhale target. Electrophoretic mobility shift assays (EMSA) idagwiritsidwa ntchito kutsimikizira kuyanjana kwa puloteni ya SlMYB ndi olimbikitsa majini target a SlMYB. Mwachidule, cholinga cha kafukufukuyu chinali kulimbikitsa kupanga kwa triterpene pogwiritsa ntchito PBZ ndikupeza MYB transcription factor (SlMYB) yomwe imalamulira mwachindunji majini a triterpene biosynthetic kuphatikiza MVD, IDI, ndi FDPS mu S. lonicericola poyankha kuyambitsa kwa PBZ.
Kuyambitsa kwa IAA ndi PBZ kunawonjezera kwambiri kupanga kwa triterpenoid mu honeysuckle, koma mphamvu ya PBZ inali yodziwika bwino. Chifukwa chake, PBZ idapezeka kuti ndiyo inducer yabwino kwambiri yokhala ndi kuchuluka kowonjezera kwa 100 mg/L, komwe kuyenera kufufuzidwa kwina.